GhBRX.1, GhBRX.2, and GhBRX4.3 improve resistance to salt and cold stress in upland cotton

• DOI: 10.3389/fpls.2024.1353365
• Publication Date: 2024-02-09T05:07:18Z
• Authors: Wei Wei; Jisheng Ju; Xueli Zhang; Pingjie Ling; Jin Luo; Ying Li; Wenjuan Xu; Junji Su; Xianliang Zhang; Caixiang Wang
• Keywords: Gossypium

Introduction Abiotic stress during growth readily reduces cotton crop yield. The different survival tactics of plants include the activation numerous response genes, such as BREVIS RADIX ( BRX ). Methods In this study, gene family upland was identified and analyzed by bioinformatics method, three salt-tolerant cold-resistant GhBRX genes were screened. expression GhBRX.1 , GhBRX.2 GhBRXL4.3 in silenced virus-induced silencing (VIGS) technique. physiological biochemical indexes related stress-response detected before after silencing. effects on salt cold resistance further verified. Results discussion We discovered 12, 6, 6 Gossypium hirsutum raimondii arboreum respectively. Chromosomal localization indicated that retention loss homologous chromosomes did not have a clear preference for subgenomes. Collinearity analysis suggested segmental duplications main force amplification. are highly expressed roots, is also strongly pistil. Transcriptome data qRT‒PCR validation showed abiotic induced . Under low-temperature conditions, activities superoxide dismutase (SOD), peroxidase (POD) catalase (CAT) content soluble sugar chlorophyll decreased GhBRX.1- GhBRX.2- -silenced compared with those control (TRV: 00). Moreover, -, - exhibited greater malondialdehyde (MDA) levels than plants. marker GhSOS1 GhSOS2 GhNHX1 GhCIPK6 GhBIN2 GhSnRK2.6 GhHDT4D GhCBF1 GhPP2C ) significantly target following exposure to stress. These results imply may be regulators responses cotton.