The modified activity of prolyl 4 hydroxylases reveals the effect of arabinogalactan proteins on changes in the cell wall during the tomato ripening process

• DOI: 10.3389/fpls.2024.1365490
• Publication Date: 2024-03-20T04:46:02Z
• Authors: Nataliia Kutyriev...; Agata Leszczuk; Lamia Ezzat; Dimitris Kaloudas; Adrian Zając; Monika Szymańska-...; Tomasz Skrzypek; Afroditi Krokida; Khansa Mekkaoui; Evangelia Lamprop...; Panagiotis Kalaitzis; Artur Zdunek
• Keywords: Arabinogalactan

Arabinogalactan proteins (AGPs) are proteoglycans with an unusual molecular structure characterised by the presence of a protein part and carbohydrate chains. Their specific properties at different stages fruit ripening programme make AGPs unique markers this process. An important function is to co-form amorphous extracellular matrix in cell wall-plasma membrane continuum; thus, changes these molecules can determine distribution other components. The aim current work was characterise localisation during process transgenic lines silencing overexpression SlP4H3 genes (prolyl 4 hydroxylase 3). objective accomplished through comprehensive comparative situ ex analyses from wild-type plants ripening. experiment showed that prolyl hydroxylases (P4H3) activity affected content progress their modifications ongoing analysis confirmed high weights (120–60 kDa) all examined stages, but changed pattern features found last compared WT. In addition AGP changes, morphological tissue alterations spatio-temporal subcellular level were detected progression highlights impact on wall associated