Systematic characterization of gene families and functional analysis of PvRAS3 and PvRAS4 involved in rosmarinic acid biosynthesis in Prunella vulgaris

• DOI: 10.3389/fpls.2024.1374912
• Publication Date: 2024-05-01T05:09:21Z
• Authors: Chao Yan; Caili Li; Maochang Jiang; Yayun Xu; Sixuan Zhang; Xiangling Hu; Yuhang Chen; Shanfa Lu
• Keywords: Rosmarinic acid; Prunella vulgaris

Prunella vulgaris is an important material for Chinese medicines with rosmarinic acid (RA) as its index component. Based on the chromosome-level genome assembly we obtained recently, 51 RA biosynthesis-related genes were identified. Sequence feature, gene expression pattern and phylogenetic relationship analyses showed that 17 of them could be involved in biosynthesis. In vitro enzymatic assay PvRAS3 catalyzed condensation p -coumaroyl-CoA caffeoyl-CoA pHPL DHPL. Its affinity toward was higher than caffeoyl-CoA. PvRAS4 lower PvRAS3. UPLC LC-MS/MS existence RA, 4-coumaroyl-3’,4’-dihydroxyphenyllactic acid, 4-coumaroyl-4’-hydroxyphenyllactic caffeoyl-4’-hydroxyphenyllactic P. . Generation analysis pvras3 homozygous mutants significant decrease increase DHPL pHPL. It suggests main enzyme catalyzing acyl donors acceptors during The role appears minor. results provide information quality control medicinal materials.