Comparison of transcriptome and metabolome analysis revealed cold-resistant metabolic pathways in cucumber roots under low-temperature stress in root zone

• DOI: 10.3389/fpls.2024.1413716
• Publication Date: 2024-09-09T04:52:46Z
• Authors: Shijun Sun; Yan Yang; Shuiyuan Hao; Ye Liu; Xin Zhang; Pudi Yang; Xudong Zhang; Yusong Luo
• Keywords: cold-resistant; Plant culture; metabolome; Plant Science; root; cucumber; transcriptome; SB1-1110

IntroductionLow ground temperature is a major factor limiting overwintering in cucumber cultivation facilities in northern alpine regions. Lower temperatures in the root zone directly affect the physiological function of the root system, which in turn affects the normal physiological activity of plants. However, the importance of the ground temperature in facilities has not attracted sufficient attention. MethodsTherefore, this study tested the cucumber variety Jinyou 35 under three root zone temperatures (room temperature, 20–22°C; suboptimal temperature, 13– 15°C; and low temperature, 8–10°C) to investigated possible cold resistance mechanisms in the root of cucumber seedlings through hormone, metabolomics, and transcriptomics analyses.Results and discussionThe results showed that cucumber roots were subjected to chilling stress at different temperatures. Hormone analysis indicated that auxin content was highest in the roots. Jasmonic acid and strigolactone participated in the low-temperature stress response. Auxin and jasmonate are key hormones that regulate the response of cucumber roots to low temperatures. Phenolic acid was the most abundant metabolite in cucumber roots under chilling stress. Additionally, triterpenes may play an important role in chilling resistance. Differentially expressed genes and metabolites were significantly enriched in benzoxazinoid biosynthesis in the room temperature vs. suboptimal temperature groups and the room temperature vs. low temperature groups. Most differentially expressed transcription factor genes in AP2/ERF were strongly induced in cucumber roots by both suboptimal and low-temperature stress conditions. These results provide guidance for the cultivation of cucumber in facilities.