Biological responses of an elite centipedegrass [Eremochloa ophiuroides (Munro) Hack.] cultivar (Ganbei) to carbon ion beam irradiation

• DOI: 10.3389/fpls.2024.1433121
• Publication Date: 2024-09-18T04:46:17Z
• Authors: Yuan Zhang; Haoran Wang; Yan Du; Ling Zhang; Xiaohui Li; Hailin Guo; Jianxiu Liu; Libin Zhou; Xin Xu; Jianjian Li
• Keywords: Germ plasm; Mutation Breeding

Carbon ion beam irradiation (CIBI) is a highly efficient mutagenesis for generating mutations that can be used to expand germplasm resources and create superior new germplasm. The study investigated the effects of different doses CIBI (50 Gy, 100 150 200 Gy 300 Gy) on seed germination seedling survival, morphological physiological traits an elite centipedegrass cultivar Ganbei. results showed greater than 50 cause inhibition germination, semi-lethal dose (LD50) around 90 treated seeds A carbon beam-mutagenized population was generated from Ganbei, with dosages Gy. More ten types phenotypic variations novel mutants heritable tendencies mainly including putative stolon number, length diameter, internode length, leaf width, chlorophyll content, growth rate, aboveground tissue dry weight, sward height were identified. While total sugar content plants irradiated no obvious change in all treatments as compared control, crude protein displayed significant reduction at high-dose treatment Genetic polymorphism detected mutagenized using SSR-PCR analysis, suggesting caused alteration larger fragments DNA sequence. As result, preliminary batch screened this study. In summary, effective way developing wider variation, treating dosage ~100 could mutation breeding.