Identification of 39 stripe rust resistance loci in a panel of 465 winter wheat entries presumed to have high-temperature adult-plant resistance through genome-wide association mapping and marker-assisted detection

• DOI: 10.3389/fpls.2024.1514926
• Publication Date: 2025-01-07T07:48:44Z
• Authors: Fangjie Yao; Meinan Wang; Deven R. See; Ennian Yang; Guoyue Chen; Xianming Chen
• Keywords: Stripe rust; Identification; Winter wheat; Association mapping; Rust (programming language); Genome-wide Association Study

Stripe rust of wheat is a serious disease caused by Puccinia striiformis f. sp. tritici (Pst). Growing resistant cultivars the most preferred approach to control disease. To identify genotypes with quantitative trait loci (QTL) for durable resistance stripe rust, 465 winter entries that were presumed have high-temperature adult-plant (HTAP) used in this study. In greenhouse seedling tests seven Pst races, 16 all tested races. The also phenotyped responses at stage under natural infection multiple field locations from 2018 2021 Washington state, and 345 found stable resistance. contrast susceptibility indicated predominantly HTAP panel. durability was demonstrated subset 175 2007 2021. genotyped through genotyping multiplexed sequencing single-nucleotide polymorphism (SNP) markers. Combining response SNP marker data, genome-wide association study (GWAS) conducted, resulting 143 marker-trait associations, which 28 QTL detected least two races or environments identified, including all-stage 21 These each explained 6.0% 40.0% phenotypic variation. Compared previously reported Yr genes based on their genomic positions, five identified as new. A total 10 user-friendly Kompetitive allele specific PCR (KASP) markers developed eight loci. addition, molecular detect 13 genes/QTL, GWAS analyses, frequencies ranged 0.86% 88.17% genotypes, genes/QTL KASP should be useful develop long-lasting rust.