Identification and functional characterization of the RPP13 gene family in potato (Solanum tuberosum L.) for disease resistance

• DOI: 10.3389/fpls.2024.1515060
• Publication Date: 2025-01-20T07:20:22Z
• Authors: Baoqi Yuan; Chuang Li; Qingfeng Wang; Qi Yao; Xiaowei Guo; Yuhang Zhang; Zhongwei Wang
• Keywords: Solanum tuberosum; Functional divergence

Potato ( Solanum tuberosum L.), as the world’s fourth largest food crop, plays a crucial role in ensuring security through its disease resistance. The RPP13 gene family is known to play pivotal plant resistance responses; however, specific functions potato remain unclear. In this study, we conducted first comprehensive identification and analysis of 28 members potato, examining their structures, chromosomal locations, expression patterns, functional characteristics. Gene structure revealed that most contain typical CC-NBS-LRR domains, with exon numbers ranging from 1 6. Phylogenetic grouped these genes into four evolutionary clades, indicating high level conservation. Cis-regulatory element identified promoter region StRPP13-26 enriched pathogen-responsive elements such WUN-motif MYC, suggesting potential defense. Expression pattern showed StRPP13-8 , StRPP13-10 StRPP13-23 are highly expressed various tissues, involvement basic physiological functions, whereas StRPP13-6 StRPP13-25 mainly induced under pathogen infection conditions. Transcriptome qRT-PCR analyses further divergence response scab disease. Notably, StRPP13-11 was significantly downregulated both resistant susceptible cultivars, early stages recognition. Subcellular localization experiments protein localized chloroplast. Combined transcriptome-based enrichment analysis, finding implies may participate defense by regulating photosynthesis-related dynamic balance reactive oxygen species within This study provides new insights mechanisms, offering valuable genetic resources theoretical support for future disease-resistant breeding programs.