Extracellular vesicles derived from fibroblasts induced with or without high glucose exert opposite effects on wound healing and angiogenesis

• DOI: 10.3389/fsurg.2022.1065172
• Publication Date: 2022-11-28T05:12:42Z
• Authors: Xiaowei Bian; Bingmin Li; Haowen Tang; Qiankun Li; Wenzhi Hu; Qian Wei; Kui Ma; Yuguang Yang; Haihong Li; Xiaobing Fu; Cuiping Zhang
• Keywords: angiogenesis; 0303 health sciences; 03 medical and health sciences; RD1-811; fibroblasts; wound healing; Surgery; extracellular vesicles; endothelial cells; high glucose; 3. Good health

Background Communication between fibroblasts and endothelial cells is essential for skin wound repair regeneration. Extracellular vesicles (EVs) are crucial intracellular communication by transporting active molecules. However, whether EVs derived from diabetic can perform the nomal function unclear. Here, we compared effects of human (HSFs) induced with or without HG on angiogenic healing. Methods We first collected HSFs cultured normal glucose concentration (NG-EVs) (HG-EVs) applied them to treat umbilical vein (HUVECs). The were divided into three groups: control group, NG-EVs HG-EVs group. then examined proliferation, migration, apoptosis, tube formation HUVECs. To illustrate mechanism, expression β-catenin, GSK-3β, p-GSK-3β was detected western-blot. Finally, used wounds mice determine their role in closure. Results By DNA content detection, Annexin V/PI staining, EdU found that promoted HUVEC while exhibited an opposite effect ( p < 0.05). Scratch assay demonstrated NG-EV angiogenesis vitro , showed negative impact expressions β-catenin HUVECs enhanced decreased Additionally, vivo experiment effectively healing locally enhancing blood supply angiogenesis. In contrast, leaded delayed closure reduced Conclusion exert possibly regulating GSK-3β/β-catenin signaling pathway. This research may provide a new treatment strategy mechanism impaired diabetics.