Deep Metabolomic Profiling Reveals Alterations in Fatty Acid Synthesis and Ketone Body Degradations in Spermatozoa and Seminal Plasma of Astheno-Oligozoospermic Bulls

• DOI: 10.3389/fvets.2021.755560
• Publication Date: 2022-01-11T05:19:38Z
• Authors: Mohua Dasgupta; Arumugam Kumaresan; Kaustubh Kishor S...; Pradeep Nag; Manish Kumar Sinha; Muhammad Aslam M. K.; Gayathree Karthik...; T. S. Keshava Prasad; Prashant Kumar Modi; Tirtha Kumar Datta; Kerekoppa Ramesha; Ayyasamy Manimaran; Sakthivel Jeyakumar
• Keywords: Asthenozoospermia; Oligospermia; Semen quality

Male fertility is extremely important in dairy animals because semen from a single bull used to inseminate several thousand females. Asthenozoospermia (reduced sperm motility) and oligozoospermia concentration) are the two reasons cited for idiopathic infertility crossbred bulls; however, etiology remains elusive. In this study, using non-targeted liquid chromatography with tandem mass spectrometry-based approach, we carried out deep metabolomic analysis of spermatozoa seminal plasma derived normozoospermic astheno-oligozoospermic bulls. Using bioinformatics tools, alterations metabolites metabolic pathways between normozoospermia astheno-oligozoospermia were elucidated. A total 299 167 183 147 detected bulls, respectively. Among mapped metabolites, 75 common both groups, whereas 166 50 unique Similarly, 86 45 37 differentially expressed 62 56 significantly dysregulated spermatozoa, selenocysteine, deoxyuridine triphosphate, nitroprusside showed significant enrichment plasma, malonic acid, 5-diphosphoinositol pentakisphosphate, D-cysteine, nicotinamide adenine dinucleotide phosphate upregulated, tetradecanoyl-CoA was downregulated astheno-oligozoospermia. Spermatozoa bulls metabolism fatty acid elongation mitochondria pathways, synthesis degradation ketone bodies, pyruvate metabolism, inositol pathways. The present study revealed vital information related differences normospermic breeding It inferred that body degradations altered These results open up new avenues further research, current findings can be applied modulation identified restore motility concentration