Eugenol Improves Follicular Survival and Development During in vitro Culture of Goat Ovarian Tissue

• DOI: 10.3389/fvets.2022.822367
• Publication Date: 2022-04-28T12:13:54Z
• Authors: R. F. Silva; L. F. Lima; Anna C. A. Ferreira; A. F. B. Silva; D. R. Alves; B. G. Alves; A. C. Oliveira; Selene M. Morais; Ana Paula R. Rodr...; Regiane R. Santos; J. R. Figueiredo
• Keywords: 0301 basic medicine; 03 medical and health sciences; in-vitro culture; antioxidant; Veterinary medicine; goat; SF600-1100; Veterinary Science; preantral follicles; eugenol

This study evaluated the effects of different concentrations (10, 20, or 40 μM) eugenol (EUG 10, EUG 40), ascorbic acid (50 μg/mL; AA) anethole (300 ANE 300) on in-vitro survival and development goat preantral follicles oxidative stress in cultured ovarian tissue. Ovarian fragments from five goats were for 1 7 days Alpha Minimum Essential Medium (α-MEM+) supplemented not with AA, 300, 20 40. On day culture, when compared to MEM, addition had increased rate follicular development, as observed by a decrease proportion primordial alongside an increase normally developing follicles. Furthermore, significantly both oocyte diameters. Subsequently, three α-MEM+ 300 All tested antioxidants, except able levels reactive oxygen species tissue, but could most efficiently neutralize free radicals. tissues presence especially 40, presented significant H3K4me3 labeling, indicating silencing genes that play role inhibition activation apoptosis induction. When control tissues, intensity calreticulin labeling growing The mRNA relative expression ERP29 KDM3A was culture medium response ER experienced during culture. In conclusion, improved follicle survival, decreased ROS production, histone lysine methylation