Breast Meat Fatty Acid Profiling and Proteomic Analysis of Beijing-You Chicken During the Laying Period

• DOI: 10.3389/fvets.2022.908862
• Publication Date: 2022-06-15T06:31:56Z
• Authors: Jian Zhang; Hong Zhuang; Jing Cao; Ailian Geng; Haihong Wang; Qin Chu; Zhixun Yan; Xiaoyue Zhang; Yao Zhang; Huagui Liu
• Keywords: KEGG; Fold change

The disparity in fatty acids (FA) composition exhibits a significant impact on meat quality, however, the molecular regulatory mechanisms underlying this trait chicken are far from clear. In study, total of 45 female Beijing-You (BYC) hens, fed same diet, were collected at slaughter age 150, 300, or 450 days (D150, D300, and D450) sexual maturation stage to culling (15 birds per age). Gas chromatography-mass spectrometry (GC-MS) tandem mass tag labeling technology based liquid chromatography (TMT-LC-MS/MS) analysis strategies applied profile FA compositions compare differential expressed proteins (DEPs) between these different ages, respectively. profiling showed that increasing hen ages resulted increased contents both saturated unsaturated acids. Proteomic analyses 4,935 breast muscle with false discovery rate (FDR) < 1% 664 them differentially (fold change > 1.50 0.67 P 0.01). There 410 up- 116 down-regulated D150 vs. D300 group, 32 20 D450 72 241 group. A 57 DEPs related FA/lipid-related metabolisms obtained according enrichment Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG). These involved 21 significantly enriched ( 0.05) pathways, including well-known pathways for synthesis (metabolism, desaturation, elongation) signaling lipid metabolism (PPAR, adipocytokine, calcium, VEGF, MAPK, Wnt). addition, there existed several representative (FABP, FABP3, apoA-I, apoA-IV, apoC-III, apoB, VTG1, VTG2) regulation FA/lipid transportation. construction interaction networks indicated HADH, ACAA2, HADHA, ACSL1, CD36, CPT1A, PPP3R1, SPHK1 key core nodes. Finally, eight quantified using parallel reaction monitoring (PRM) validate results TMT analysis. expanded our understanding how laying affects meat.