Arginine Regulates Zygotic Genome Activation in Porcine Embryos Under Nutrition Restriction

• DOI: 10.3389/fvets.2022.921406
• Publication Date: 2022-06-23T09:43:01Z
• Authors: Tianrui Zhang; Yingying Zheng; Tianya Kuang; Lianyu Yang; Hailong Jiang; Heming Wang; Yicheng Zhao; Rui Han; Dongsheng Che
• Keywords: 0301 basic medicine; 2. Zero hunger; zygotic genome activation; 03 medical and health sciences; embryonic development; Veterinary medicine; energy metabolism; SF600-1100; oxidative stress; arginine; Veterinary Science; porcine; 3. Good health

Arginine has a positive effect on pre-implantation development in pigs. However, the exact mechanism by which arginine promotes embryonic is undefined. Here, single-cell RNA sequencing technology was applied to porcine vivo embryos from zygote morula stage, it found that expression of metabolism-related genes clearly changed 2-cell stage 4-cell when zygotic genome activation (ZGA) occurs embryos. Further analysis showed are significantly correlated with key ZGA genes. To determine function during ZGA, vitro fertilization were cultured PZM-3 medium (0.12 mM arginine, Control group), modified (0 Block group) and supplemented + Arg group). The results arrest rate increased group compared (P < 0.05). decreased than Meanwhile, marker SIRT1 protein group, their group. In addition, we observed glutathione (GSH), ATP levels, lipid droplet contents increased, reactive oxygen species (ROS) level Compared spermine content culture mRNA ornithine decarboxylase1 (ODC1) 0.05), those Moreover, difluoromethylornithine (an inhibitor ODC1) added inhibited. summary, our findings demonstrated may regulate under nutrition restriction promoting polyamine synthesis.