A comparative study of eight serological methods shows that spike protein-based ELISAs are the most accurate tests for serodiagnosing SARS-CoV-2 infections in cats and dogs

• DOI: 10.3389/fvets.2023.1121935
• Publication Date: 2023-01-26T08:20:39Z
• Authors: Carlos Diezma-Díaz; Gema Álvarez-García; Javier Regidor-Ce...; Guadalupe Miró; Sergio Villanueva...; María Dolores Pérez; María Teresa Verde; Patricia Galán-Malo; Alejandro Brun; Sandra Moreno; Rocío Checa; Ana Montoya; Wesley C. Van Voo...; Luis Miguel Orteg...
• Keywords: Pandemic; Coronavirus

Introduction Coronavirus disease 2019 (COVID-19) is an infectious zoonotic caused by SARS-CoV-2. Monitoring the infection in pets recommended for human surveillance, prevention, and control since virus can spread from people to animals during close contact. Several diagnostic tests have been adapted humans animals, but limited data on validation process are available. Methods Herein, first comparative study of six “ house ” two commercial serological developed monitor SARS-CoV-2 was performed with a well-coded panel sera (61 cat 74 dog sera) conservative criterion (viral seroneutralisation and/or RT–qPCR results) as reference. Four based either RBD fragment spike protein (RBD-S) or N-terminal nucleoprotein (N) were time. The analytical specificity (ASp) those that showed best performance assessed. included analysis obtained pre-pandemic cats dogs infected other coronaviruses determine Sp (17 41 sera). Results discussion ELISAS S serosurveillance studies (RBD-S SALUVET ELISA, ELISA COVID UNIZAR INgezim ® 19 VET) (INgezim VET RBD-S ELISA). These higher sensitivity (Se) DSp (>90%) than dogs. When prior pandemic analyzed N ELISAs VET, few cross reactors no reactions detected when protein, respectively. In contrast, number increased test protein. Thus, use discarded serodiagnosis purposes. results revealed most accurate each species. Further should attempt improve