Impact of negative energy balance and postpartum diseases during the transition period on oocyte quality and embryonic development in dairy cows

• DOI: 10.3389/fvets.2023.1328700
• Publication Date: 2024-01-05T05:47:48Z
• Authors: Idil Serbetci; L. Antonio Gonzál...; Carolina Herrera; Iulian Ibanescu; Muhittin Tekin; Manuel Melean; Fumie Magata; Eleni Malama; Heinrich Bollwein; Dragos Scarlet
• Keywords: Embryo quality; Subclinical infection

Transition period is a critical time for dairy cows because large proportion of clinical and subclinical diseases are observed in the first month after parturition. Occurrence negative energy balance associated with depressed immunity these conditions can affect oocyte quality further embryonic development. The aim this study was to assess effects balance-associated disorders on vitro embryo production (IVP) cattle. We hypothesized that metabolic and/or inflammatory have effect developmental competence morphokinetic parameters resulting embryos. conducted 30 lactating Holstein-Friesian which were assigned into four groups: healthy (HEAL, n = 6), disease (META, 8), (INFL, or combined (COMB, 8). Ovum pick-up (OPU) performed twice weekly all over weeks ( 8 OPU sessions/cow) starting fifth week postpartum, collected oocytes subjected routine IVP. Donor’s health status did not number oocytes/OPU recovery rate p > 0.05). 1 from INFL COMB lower compared HEAL < Also, percentage embryos reduced META Cleavage, blastocyst hatching rates similar among groups Presence required by zygotes reach specific stages, as recorded means time-lapse monitoring. Nevertheless, there higher probability direct cleavage IVF those In conclusion, derived diagnosed during transition showed but potential morphokinetics when cows. These results shed light consequences development might be important transfer programs.