Prevalence and characterization of antimicrobial-resistant Escherichia coli in chicken meat from wet markets in Hong Kong

• DOI: 10.3389/fvets.2024.1340548
• Publication Date: 2024-01-15T04:21:27Z
• Authors: F. M. Yasir Hasib; Ioannis Magouras; Sophie St-Hilaire; Surya Paudel; Maedeh Kamali; Kittitat Lugsomya; Hoi Kiu Lam; Ibrahim Elsohaby; Patrick Butaye; Omid Nekouei
• Keywords: Broth microdilution

Given the close contact between animals, animal products, and consumers in wet markets, fresh meat products are considered a potential source disseminator of antimicrobial-resistant (AMR) bacteria near end food chain. This cross-sectional study was conducted to estimate prevalence select AMR- E. coli chicken collected from markets Hong Kong determine target genes associated with observed resistance phenotypes. Following stratified random sampling design, 180 half-chickens were purchased 29 across 2022 immediately processed. After incubation, selective isolation performed for extended-spectrum β-lactamase producing (ESBL), carbapenem-resistant (CRE), colistin-resistant (CSR) . The bacterial isolates identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Disc Diffusion used susceptibility ESBL- CRE- isolates. broth microdilution method minimum inhibitory concentration CSR- Targeted then detected by PCR. estimated at 88.8% (95% CI: 83.4–93.1%) 6.7% 3.5–11.4%), respectively. No isolate detected. bla CTX-M-1 gene most common group isolated (80%), followed TEM (63.7%); no SHV Forty-five percent had simultaneously. mcr-1 all 12 CSR Of samples, 59 Mainland China, 121 locally sourced. There statistically significant difference two sources. Our findings can be inform safety risk assessments set stage adopting targeted control mitigation measures tailored local markets.