Effects of clove oil concentrations on blood chemistry and stress-related gene expression in Siamese fighting fish (Betta splendens) during transportation

• DOI: 10.3389/fvets.2024.1392413
• Publication Date: 2024-05-22T14:05:47Z
• Authors: Chanoknan Sintuprom; Wirawan Nuchchanart; Sahabhop Dokkaew; Chak Aranyakanont; Raveewan Ploypan; Andrew P. Shinn; Ratchakorn Wongwa...; Nguyen Dinh-Hung; Ha Thanh Dong; Satid Chatchaiphan
• Keywords: transportation; 0301 basic medicine; stress; 0303 health sciences; Veterinary medicine; clove oil; SF600-1100; Veterinary Science; Betta splendens; animal welfare

Siamese fishing fish ( Betta splendens ) or betta are usually subjected to a special method of transportation for global trade, where they individually conveyed in plastic bags containing just enough water cover their bodies. This study aimed investigate the effects on stress response by measuring hematological values, hormone levels, glucose and stress-related gene expression. (average body weight 1.91 ± 0.42 g; n = 30) were exposed simulated transport volume 40 mL 12, 24, 48 h. Baseline levels (pre-transport) measured prior experiment. The control group was transported using without adding clove oil. Two treatment groups with addition 1 3 mg/L concentrations oil, respectively. results revealed that can be factor affects quality. pH dissolved oxygen significantly lower than baseline, while nitrite total ammonia increased. Correlating responses, increasing red blood cell counts, plasma cortisol up-regulating expression genes, including HSP70 , GR MR HIF-1α . oil found reduce during simulation, as evidenced reduction these parameters. Conversely, concentration increased after 12 h transport, up-regulated process stimulates but could mitigate this promote animal welfare transportation.