Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation

• DOI: 10.3389/ti.2023.11518
• Publication Date: 2023-09-08T04:11:26Z
• Authors: Javier Serradilla; Ane Miren Andrés ...; Paloma Talayero; Paula Burgos; Mariana Machuca; Onys Camps Ortega; María Teresa Vallejo; Francisco Javier ...; Alba Bueno; Alba Sánchez; Cristina Zambrano; Carlos Andrés De ...; Olaia Rodríguez; Carlota Largo; Pilar Serrano; Gerardo Prieto Bo...; Esther Ramos; Manuel López Sant...; Pablo Stringa; Francisco Hernández
• Keywords: INTESTINAL TRANSPLANTATION; Graft Rejection; ORGAN PROCUREMENT; Liver Cirrhosis and Associated Complications; Swine; Urology; Health Archive; Ischemia; https://purl.org/becyt/ford/3.1; Health Sciences; DCD; Animals; Humans; https://purl.org/becyt/ford/3; Internal medicine; Liver Transplantation and Graft Survival Analysis; Transplantation; Hepatology; Gastroenterology; Kidney Transplantation; Tissue Donors; Machine Perfusion; Perfusion; SOLID ORGAN TRANSPLANTATION; Reperfusion Injury; Reperfusion; Medicine; Surgery

Considering recent clinical and experimental evidence, expectations for using DCD-derived intestines have increased considerably. However, more knowledge about DCD procedure long-term results after intestinal transplantation (ITx) is needed. We aimed to describe in detail a ITx normothermic regional perfusion (NRP) preclinical model. Small bowel was obtained from pigs donors 1 h of NRP transplanted the recipients. Graft Intestinal samples were during transplantation. Ischemia-reperfusion injury (Park-Chiu score), graft rejection absorptive function evaluated. Seven 8 procedures with successful (87.5%), good reperfusion an excellent recovery recipient. The architecture grafts well conserved NRP. After initial damage Park-chiu score 4, all recovered ischemia-reperfusion, no or very subtle alterations 2 days ITx. Most recipients (71.5%) did not show signs rejection. Only two cases demonstrated histologic mild 7 Interestingly showed capacity. study's support viability NRP, contributing evidence use