Effects of Resistance Training on the Redox Status of Skeletal Muscle in Older Adults

• DOI: 10.3390/antiox10030350
• Publication Date: 2021-02-27T02:02:34Z
• Authors: Paulo H. C. Mesquita; Donald A. Lamb; Joshua S. Godwin; Shelby C. Osburn; Bradley A. Ruple; Johnathon H. Moore; Christopher G. Vann; Kevin W. Huggins; Andrew D. Fruge; Kaelin C. Young; Andreas N. Kavazis; Michael D. Roberts
• Keywords: Glutathione reductase; KEAP1

The aim of this study was to investigate the effects resistance training (RT) on redox status skeletal muscle in older adults. Thirteen males aged 64 ± 9 years performed full-body RT 2x/week for 6 weeks. Muscle biopsies were obtained from vastus lateralis prior and following RT. mRNA, protein, enzymatic activity levels various endogenous antioxidants determined. In addition, 4-hydroxynonenal protein carbonyls determined as markers oxidative damage. Protein heat shock proteins (HSPs) also quantified. increased mRNA all assayed antioxidant genes, albeit either did not change or decreased. total capacity, catalase, glutathione reductase activities, decreased peroxidase activity. Lipid peroxidation HSP60 summary, weeks damage enzyme activities. Our results suggest adult responses involve multi-level (transcriptional, post-transcriptional, post-translational) control muscle.