Cimicifugae Rhizoma Extract Attenuates Oxidative Stress and Airway Inflammation via the Upregulation of Nrf2/HO-1/NQO1 and Downregulation of NF-κB Phosphorylation in Ovalbumin-Induced Asthma

• DOI: 10.3390/antiox10101626
• Publication Date: 2021-10-15T17:35:47Z
• Authors: Je-Oh Lim; Kwang Hoon Song; Ik Soo Lee; Se-Jin Lee; Woong-Il Kim; So-Won Pak; In-Sik Shin; Taesoo Kim
• Keywords: Allergic Inflammation

Cimicifugae Rhizoma has been used as a medicinal herb for fever, pain, and inflammation in East Asia. We conducted this study because the effect of extract (CRE) on allergic asthma not yet evaluated. To induce airway inflammation, we intraperitoneally injected ovalbumin (OVA) mixed with aluminum hydroxide into mice twice at intervals 2 weeks (Days 0 14) then inhaled them thrice 1% OVA solution using nebulizer 21 to 23). CRE (30 100 mg/kg) was administered orally daily 6 days 18 The showed remarkable reduction mg/kg CRE, evidenced by decreased inflammatory cell counts, pro-inflammatory cytokine levels, OVA-specific immunoglobulin E level, hyperresponsiveness, production mucus. Additionally, these effects were involved enhancement heme oxygenase-1 (HO-1), NAD(P)H: quinone oxidoreductase (NQO1), nuclear factor erythroid 2-related (Nrf2) expression factor-κB (NF-κB) phosphorylation matrix metalloproteinase-9 expression. Our findings indicated that effectively protected against OVA-induced oxidative stress via upregulation Nrf2/HO-1/NQO1 signaling downregulation NF-κB caused OVA.