An SPM-Enriched Marine Oil Supplement Shifted Microglia Polarization toward M2, Ameliorating Retinal Degeneration in rd10 Mice

• DOI: 10.3390/antiox12010098
• Publication Date: 2023-01-02T09:51:22Z
• Authors: Lorena Olivares-G...; Sheyla Velasco; Idoia Gallego; Marina Esteban-Me...; Gustavo Puras; Carlos Loucera; Alicia Martínez-R...; María Peña-Chilet; José Luis Pedraz; Regina Rodrigo
• Keywords: Electroretinography

Retinitis pigmentosa (RP) is the most common inherited retinal dystrophy causing progressive vision loss. It accompanied by chronic and sustained inflammation, including M1 microglia activation. This study evaluated effect of an essential fatty acid (EFA) supplement containing specialized pro-resolving mediators (SPMs), on degeneration activation in rd10 mice, a model RP, as well LPS-stimulated BV2 cells. The EFA was orally administered to mice from postnatal day (P)9 P18. At P18, electrical activity retina examined electroretinography (ERG) innate behavior response light were measured. Retinal studied via histology TUNEL assay immunolabeling. Microglia polarization (M1/M2) assessed flow cytometry, qPCR, ELISA histology. Redox status analyzed measuring antioxidant enzymes markers oxidative damage. Interestingly, ameliorated dysfunction improving ERG recording sensitivity light, reducing photoreceptor cell reduced inflammation attenuating inducing shift M2 phenotype mouse retinas also stress lipid peroxidation carbonylation. These findings could open up new therapeutic opportunities based resolving with oral supplementation SPMs such supplement.