Multi-Omics Reveals Disrupted Immunometabolic Homeostasis and Oxidative Stress in Adipose Tissue of Dairy Cows with Subclinical Ketosis: A Sphingolipid-Centric Perspective

• DOI: 10.3390/antiox13050614
• Publication Date: 2024-05-17T16:02:23Z
• Authors: Huiying Zhao; Liuxue Li; Jian Tan; Ying Wang; Ao Zhang; Yuchao Zhao; Linshu Jiang
• Keywords: Sphingolipid; Ketosis; Homeostasis; Subclinical infection; Omics

Ketosis, especially its subclinical form, is frequently observed in high-yielding dairy cows and linked to various diseases during the transition period. Although adipose tissue plays a significant role development of metabolic disorders, exact impact on emergence ketosis (SCK) still poorly understood. The objectives this study were characterize compare profiling transcriptome lipidome blood between SCK healthy investigate potential correlation disorders lipid metabolism. We obtained samples from (CON, n = 8, β-hydroxybutyric acid concentration < 1.2 mmol/L) ketotic (SCK, 1.2–3.0 for analyzing biochemical parameters, transcriptome, lipidome. found that serum levels nonesterified fatty acids, malonaldehyde, amyloid A protein, IL-1β, IL-6 higher than CON cows. Levels adiponectin total antioxidant capacity top enriched pathways whole associated with immune inflammatory responses sphingolipid metabolism, respectively. accumulation ceramide sphingomyelin was paralleled by an increase genes related biosynthesis, lipolysis, inflammation decrease catabolism, lipogenesis, production, enzyme systems. Increased concentrations correlated reduced insulin sensitivity. current results indicate profile altered certain species correlate health. Our research suggests disruptions metabolism could be crucial progression SCK, exacerbating conditions such as resistance, increased inflammation, oxidative stress, providing biomarker novel target nutritional manipulation pharmacological therapy.