New Lipophenol Antioxidants Reduce Oxidative Damage in Retina Pigment Epithelial Cells

• DOI: 10.3390/antiox7120197
• Publication Date: 2018-12-19T17:12:44Z
• Authors: Espérance Moine; Philippe Brabet; Laurent Guillou; Thierry Durand; Joseph Vercauteren; Céline Crauste
• Keywords: Lipofuscin

Age-related macular degeneration (AMD) is a multifactorial pathology and its progression exacerbated by oxidative stress. Oxidation photo-oxidation reactions modify lipids in retinal cells, contribute to tissue injury, lead the formation of toxic adducts. In particular, autofluorescent pigments such as N-retinylidene-N-retinylethanolamine (A2E) accumulate lipofuscin pigment epithelial production additional reactive oxygen species (ROS), cell degeneration. an effort develop efficient antioxidants reduce damage caused lipid oxidation, various natural polyphenols were structurally modified increase their lipophilicity (lipophenols). this study, resveratrol, phloroglucinol, quercetin catechin selected conjugated polyunsaturated fatty acids (PUFAs) using classical chemical strategies or enzymatic reactions. After screening for cytotoxicity, capacity synthesized lipophenols ROS was evaluated ARPE-19 cells subjected H2O2 treatment dichlorofluorescein diacetate probe. The positions PUFA on polyphenol core appear influence antioxidant effect. addition, two lipophenolic derivatives highlight potency protecting against A2E toxicity. Quercetin linoleic α-linolenic acid promising lipophilic antioxidants, they protected from A2E-induced death more effectively than parent polyphenol, quercetin.