Biocatalytic Synthesis of Fluorescent Conjugated Indole Oligomers

• DOI: 10.3390/bioengineering1040246
• Publication Date: 2014-12-03T15:27:09Z
• Authors: Ryan Bouldin; Ankita Singh; Michael Magaletta; Sean Connor; Jayant Kumar; Ramaswamy Nagarajan
• Keywords: Oligomer; Biocompatibility

Fluorescent conjugated materials exhibiting reasonable biocompatibility that are capable of interacting with biological molecules interest for bio-sensing and imaging applications. Traditional approaches do not allow the synthesis in presence biologically relevant substrates. Further polymers synthesized using conventional methods doped fluorescent. Here we explore possibility synthesizing fluorescent oligomers indole enzymes as catalyst under mild conditions. The peroxidase catalyzed coupling reaction presented here creates a photoluminescent material allows direct utilization (without purification separation dopant) biosensing polymerization proceeds smoothly just deionized water ethanol. Monitoring absorption fluorescence spectra over one hour shows concentration both absorbing emitting species grows steadily time. anionic buffers templates is shown to effectively retard development light instead leads formation an electrically polymer. Structural characterization through FTIR ¹H-NMR analysis suggests oligomer coupled 2 3 positions on ring.