Modulation of Macrophage Activity by Pulsed Electromagnetic Fields in the Context of Fracture Healing

• DOI: 10.3390/bioengineering8110167
• Publication Date: 2021-11-01T15:47:38Z
• Authors: Yangmengfan Chen; Maximilian M. Menger; Benedikt J. Braun; Sara Schweizer; Caren Linnemann; Karsten Falldorf; Michael Ronniger; Hongbo Wang; Tina Histing; Andreas K. Nussler; Sabrina Ehnert
• Keywords: Proinflammatory cytokine

Delayed fracture healing and non-unions impose an enormous burden on individuals society. Successful requires tight communication between immune cells bone cells. Macrophages can be found in all phases. Due to their high plasticity long life span, they represent good target for modulation. In the past, extremely low frequency pulsed electromagnet fields (ELF-PEMFs) have been shown exert cell-specific effects depending field conditions. Thus, aim was identify specific ELF-PEMFs able modulate macrophage activity indirectly promote mesenchymal stem/stromal cell (SCP-1 cells) function. After a blinded screening of 22 different ELF-PEMF, two (termed A B) were further characterized as diversely affected These similar fundamental frequencies (51.8 Hz 52.3 Hz) but are emitted groups pulses or rather send-pause intervals. exposed showed pro-inflammatory function, represented by increased levels phospho-Stat1 CD86, accumulation ROS, secretion cytokines. contrast, macrophages B anti-inflammatory pro-healing functions, Arginase I, cytokines, growth factors known induce processes. The conditioned medium from both favored migration SCP-1 cells, effect stronger B. Furthermore, B, not A, stimulated expression extracellular matrix genes i.e., COL1A1, FN1, BGN. summary, our data show that may affect knowing conditions underlying mechanisms bears great potential adjuvant treatment responses during pathologies, e.g., healing.