Guided Self-Assembly of ES-Derived Lung Progenitors into Biomimetic Tube Structures That Impact Cell Differentiation

• DOI: 10.3390/bioengineering8120209
• Publication Date: 2021-12-10T13:17:58Z
• Authors: John P. Soleas; Linwen Huang; Elisa D’Arcangelo; Maria Cristina No...; Thomas K. Waddell; Alison P. McGuigan; Golnaz Karoubi
• Keywords: Decellularization; Directed differentiation

Chemically directed differentiation of pluripotent stem cells (PSCs) into defined cell types is a potent strategy for creating regenerative tissue models and therapies. In vitro observations suggest that physical cues can augment differentiation. We recently demonstrated confining human PSC-derived lung progenitor in tube with diameter mimics those observed during development results the alteration towards SOX2-SOX9+ cells. Here we set out to assess robustness this geometric confinement effect respect different culture parameters order explore corresponding changes morphometry determine feasibility using such an approach enhance protocols. Culture polydimethylsiloxane (PDMS) tubes reliably induced self-organization structures was insensitive variety extracellular matrix coatings. Cellular morphology status were found be sensitive cultured within but not seeding density. These data impose constraints on cells, homogenize cellular morphology, influence fate status.