Characterization and Inkjet Printing of an RNA Aptamer for Paper-Based Biosensing of Ciprofloxacin

• DOI: 10.3390/bios9010007
• Publication Date: 2019-01-03T08:36:30Z
• Authors: Jeannine Jaeger; Florian Groher; Jacqueline Stamm; Dieter Spiehl; Johannes Braun; Edgar Dörsam; Beatrix Suess
• Keywords: Aptamer; Riboswitch

The excessive use of antibiotics in food-producing animals causes a steady rise multiple antibiotic resistance foodborne bacteria. Next to sulfonamides, the most common groups are fluoroquinolones, aminoglycosides, and ß-lactams. Therefore, there is need for quick, efficient, low-cost detection procedure antibiotics. In this study, we propose an inkjet-printed aptamer-based biosensor developed fluoroquinolone ciprofloxacin. Due their extraordinary high affinity specificity, aptamers already widely used various applications. Here present ciprofloxacin-binding RNA aptamer by systematic evolution ligands exponential enrichment (SELEX). We characterized secondary structure determined KD 36 nM that allow contamination relevant range. demonstrate can be inkjet-printed, dried, resolved while keeping functionality consistently intact. With proof concept, paving way potential range additional aptamer-based, printable biosensors.