Geniposide Ameliorated Dexamethasone-Induced Cholesterol Accumulation in Osteoblasts by Mediating the GLP-1R/ABCA1 Axis

• DOI: 10.3390/cells10123424
• Publication Date: 2021-12-07T03:18:42Z
• Authors: Yizhou Zheng; Yaosheng Xiao; Di Zhang; Shanshan Zhang; Jing Ouyang; Linfu Li; Weimei Shi; Rui Zhang; Hai Liu; Qi Jin; Zhixi Chen; Daohua Xu; Longhuo Wu
• Keywords: ABCA1; Article; Dexamethasone; Glucagon-Like Peptide-1 Receptor; geniposide; Mice; Animals; Iridoids; GLP-1R; Osteoblasts; QH573-671; Eucommiaceae; cholesterol; Cell Differentiation; 3T3 Cells; osteoporosis; glucocorticoid; cholesterol; geniposide; GLP-1R; ABCA1; osteoporosis; Rats; 3. Good health; Disease Models, Animal; Cholesterol; Gene Expression Regulation; Osteoporosis; glucocorticoid; Cytology; ATP Binding Cassette Transporter 1; Signal Transduction

Overexposure to glucocorticoid (GC) produces various clinical complications, including osteoporosis (OP), dyslipidemia, and hypercholesterolemia. Geniposide (GEN) is a natural iridoid compound isolated from Eucommia ulmoides. Our previous study found that GEN could alleviate dexamethasone (DEX)-induced differentiation inhibition of MC3T3-E1 cells. However, whether protected against Dex-induced cholesterol accumulation in osteoblasts was still unclear.DEX used induce rat OP. Micro-CT data obtained. The ALP activity mineralization were determined by the staining assays, total intracellular ELISA kits. protein expression detected western blot.GEN ameliorated micro-structure damages cell bone trabecula rats. In cells, Dex enhanced cholesterol, which reduced proliferation differentiation. Effectively, decreased DEX-induced accumulation, differentiation, upregulated GLP-1R/ABCA1 axis. addition, ABAC1 reversed actions GEN. Treatment with Exendin9-39, GLP-1R inhibitor, abrogate protective GEN.GEN mediating axis