Evaluation of Fat Accumulation and Adipokine Production during the Long-Term Adipogenic Differentiation of Porcine Intramuscular Preadipocytes and Study of the Influence of Immunobiotics

Leptin 0301 basic medicine FAT ACCUMULATION Swine Epidemiology Physiology Beige Adipocytes long-term adipogenic differentiation (LTAD) Cell Count Brown Adipose Tissue Function and Physiology ADIPOKINES Ligands IMMUNOBIOTICS Endocrinology https://purl.org/becyt/ford/3.1 Adipocytes Andrology adipokines Internal medicine Adiposity 2. Zero hunger 0303 health sciences Adipogenesis Muscles Fatty Acids Toll-Like Receptors Rehabilitation 3. Good health Chemistry Adipose Tissue PORCINE INTRAMUSCULAR ADIPOCYTE porcine intramuscular adipocyte Medicine Adiponectin Adipose tissue Article Cell Line 03 medical and health sciences INFLAMMATION Adipokines Adipokine Health Sciences Animals The Role of Exercise in Inflammation and Oxidative Stress https://purl.org/becyt/ford/3 Obesity Biology Cell Proliferation Cell Size Inflammation QH573-671 long-term adipogenic differentiation (LTAD), inflammation Tumor Necrosis Factor-alpha Insulin resistance immunobiotics LONG-TERM ADIPOGENIC DIFFERENTIATION (LTAD) fat accumulation Adaptive Thermogenesis Inflammation and Obesity-Related Metabolic Disorders inflammation FOS: Biological sciences Stimulation Cytology
DOI: 10.3390/cells9071715 Publication Date: 2020-07-21T10:38:55Z
ABSTRACT
The degree of fat accumulation and adipokine production are two major indicators obesity that correlated with increased adipose tissue mass chronic inflammatory responses. Adipocytes have been considered effector cells for the responses due to their capacity express Toll-like receptors (TLRs). In this study, we evaluated in porcine intramuscular preadipocyte (PIP) maintained vitro differentiation over a long period without or stimulation either TNF-α TLR2-, TLR3-, TLR4-ligands. cytosolic was measured by liquid chromatography expression adipokines (CCL2, IL-6, IL-8 IL-10) were quantified RT-qPCR ELISA at several time points (0 20 days) PIP differentiation. Long-term adipogenic (LTAD) induced progressive adipocytes time. Activation TLR3 TLR4 resulted an rate into LTAD. CCL2, IL-6 significantly unstimulated during LTAD, while IL-10 remained stable studied period. An increasing trend adiponectin leptin also observed On other hand, TLRs agonists all four treatments We examined influences immunoregulatory probiotic strains (immunobiotics) on modulation using supernatants immunobiotic-treated intestinal immune LTAD cells. Immunobiotics shown strain-specific ability modulate production, adipocytes. Here, expanded utility potential application our model evaluating (20 order elucidate further insights pathobiology associated as well explore prospects immunomodulatory intervention such immunobiotics.
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