Evaluation of Fat Accumulation and Adipokine Production during the Long-Term Adipogenic Differentiation of Porcine Intramuscular Preadipocytes and Study of the Influence of Immunobiotics

• DOI: 10.3390/cells9071715
• Publication Date: 2020-07-21T10:38:55Z
• Authors: Asuka Tada; AKM Humayun Kober; Md. Aminul Islam; Manami Igata; Michihiro Takagi; Masahiko Suzuki; Hisashi Aso; Wakako Ikeda-Ohtsubo; Kazutoyo Yoda; Kenji Miyazawa; Fang He; Hideki Takahashi; Julio Villena; Haruki Kitazawa
• Keywords: Leptin; 0301 basic medicine; FAT ACCUMULATION; Swine; Epidemiology; Physiology; Beige Adipocytes; long-term adipogenic differentiation (LTAD); Cell Count; Brown Adipose Tissue Function and Physiology; ADIPOKINES; Ligands; IMMUNOBIOTICS; Endocrinology; https://purl.org/becyt/ford/3.1; Adipocytes; Andrology; adipokines; Internal medicine; Adiposity; 2. Zero hunger; 0303 health sciences; Adipogenesis; Muscles; Fatty Acids; Toll-Like Receptors; Rehabilitation; 3. Good health; Chemistry; Adipose Tissue; PORCINE INTRAMUSCULAR ADIPOCYTE; porcine intramuscular adipocyte; Medicine; Adiponectin; Adipose tissue; Article; Cell Line; 03 medical and health sciences; INFLAMMATION; Adipokines; Adipokine; Health Sciences; Animals; The Role of Exercise in Inflammation and Oxidative Stress; https://purl.org/becyt/ford/3; Obesity; Biology; Cell Proliferation; Cell Size; Inflammation; QH573-671; long-term adipogenic differentiation (LTAD), inflammation; Tumor Necrosis Factor-alpha; Insulin resistance; immunobiotics; LONG-TERM ADIPOGENIC DIFFERENTIATION (LTAD); fat accumulation; Adaptive Thermogenesis; Inflammation and Obesity-Related Metabolic Disorders; inflammation; FOS: Biological sciences; Stimulation; Cytology

The degree of fat accumulation and adipokine production are two major indicators obesity that correlated with increased adipose tissue mass chronic inflammatory responses. Adipocytes have been considered effector cells for the responses due to their capacity express Toll-like receptors (TLRs). In this study, we evaluated in porcine intramuscular preadipocyte (PIP) maintained vitro differentiation over a long period without or stimulation either TNF-α TLR2-, TLR3-, TLR4-ligands. cytosolic was measured by liquid chromatography expression adipokines (CCL2, IL-6, IL-8 IL-10) were quantified RT-qPCR ELISA at several time points (0 20 days) PIP differentiation. Long-term adipogenic (LTAD) induced progressive adipocytes time. Activation TLR3 TLR4 resulted an rate into LTAD. CCL2, IL-6 significantly unstimulated during LTAD, while IL-10 remained stable studied period. An increasing trend adiponectin leptin also observed On other hand, TLRs agonists all four treatments We examined influences immunoregulatory probiotic strains (immunobiotics) on modulation using supernatants immunobiotic-treated intestinal immune LTAD cells. Immunobiotics shown strain-specific ability modulate production, adipocytes. Here, expanded utility potential application our model evaluating (20 order elucidate further insights pathobiology associated as well explore prospects immunomodulatory intervention such immunobiotics.