Evaluation of Fat Accumulation and Adipokine Production during the Long-Term Adipogenic Differentiation of Porcine Intramuscular Preadipocytes and Study of the Influence of Immunobiotics
Leptin
0301 basic medicine
FAT ACCUMULATION
Swine
Epidemiology
Physiology
Beige Adipocytes
long-term adipogenic differentiation (LTAD)
Cell Count
Brown Adipose Tissue Function and Physiology
ADIPOKINES
Ligands
IMMUNOBIOTICS
Endocrinology
https://purl.org/becyt/ford/3.1
Adipocytes
Andrology
adipokines
Internal medicine
Adiposity
2. Zero hunger
0303 health sciences
Adipogenesis
Muscles
Fatty Acids
Toll-Like Receptors
Rehabilitation
3. Good health
Chemistry
Adipose Tissue
PORCINE INTRAMUSCULAR ADIPOCYTE
porcine intramuscular adipocyte
Medicine
Adiponectin
Adipose tissue
Article
Cell Line
03 medical and health sciences
INFLAMMATION
Adipokines
Adipokine
Health Sciences
Animals
The Role of Exercise in Inflammation and Oxidative Stress
https://purl.org/becyt/ford/3
Obesity
Biology
Cell Proliferation
Cell Size
Inflammation
QH573-671
long-term adipogenic differentiation (LTAD), inflammation
Tumor Necrosis Factor-alpha
Insulin resistance
immunobiotics
LONG-TERM ADIPOGENIC DIFFERENTIATION (LTAD)
fat accumulation
Adaptive Thermogenesis
Inflammation and Obesity-Related Metabolic Disorders
inflammation
FOS: Biological sciences
Stimulation
Cytology
DOI:
10.3390/cells9071715
Publication Date:
2020-07-21T10:38:55Z
AUTHORS (14)
ABSTRACT
The degree of fat accumulation and adipokine production are two major indicators obesity that correlated with increased adipose tissue mass chronic inflammatory responses. Adipocytes have been considered effector cells for the responses due to their capacity express Toll-like receptors (TLRs). In this study, we evaluated in porcine intramuscular preadipocyte (PIP) maintained vitro differentiation over a long period without or stimulation either TNF-α TLR2-, TLR3-, TLR4-ligands. cytosolic was measured by liquid chromatography expression adipokines (CCL2, IL-6, IL-8 IL-10) were quantified RT-qPCR ELISA at several time points (0 20 days) PIP differentiation. Long-term adipogenic (LTAD) induced progressive adipocytes time. Activation TLR3 TLR4 resulted an rate into LTAD. CCL2, IL-6 significantly unstimulated during LTAD, while IL-10 remained stable studied period. An increasing trend adiponectin leptin also observed On other hand, TLRs agonists all four treatments We examined influences immunoregulatory probiotic strains (immunobiotics) on modulation using supernatants immunobiotic-treated intestinal immune LTAD cells. Immunobiotics shown strain-specific ability modulate production, adipocytes. Here, expanded utility potential application our model evaluating (20 order elucidate further insights pathobiology associated as well explore prospects immunomodulatory intervention such immunobiotics.
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CITATIONS (4)
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