Bioinspired Mineralization of Type I Collagen Fibrils with Apatite in Presence of Citrate and Europium Ions

• DOI: 10.3390/cryst9010013
• Publication Date: 2018-12-26T09:29:54Z
• Authors: Jaime Gómez Morales; Raquel Fernández ...; Cristóbal Verdugo...; Lorenzo Degli Esp...; Francesca Oltolina; Maria Prat; Michele Iafisco; Jorge Fernando Fe...
• Keywords: Nanocrystalline material; Amorphous calcium phosphate

Synthetic nanostructured hybrid composites based on collagen and nanocrystalline apatites are interesting materials for the generation of scaffolds bone tissue engineering. In this work, mineralized fibrils were prepared in presence citrate Eu3+. Citrate is an indispensable essential structural/functional component bone. Eu3+ endows necessary luminescent features to be potentially employed as a diagnostic tool biomedical applications. The assembly mineralization performed by neutralization method, which consists adding dropwise Ca(OH)2 solution H3PO4 containing dispersed type I until neutralization. absence citrate, resultant with apatites. When was added titrant Citrate/Ca molar ratio 2 or 1, it acted inhibitor transformation amorphous calcium phosphate (ACP) apatite. addition same lead formation Eu3+–doped citrate–coated ACP/collagen fibrils. Interestingly, relative intensity luminescence lifetime latter composite superior those apatite/collagen citrate. cytocompatibility tests, evaluated 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) colorimetric assay dose–dependent manner GTL–16 human gastric carcinoma cells, MG–63 osteosarcoma cells m17.ASC, spontaneously immortalized mouse mesenchymal stem cell clone from subcutaneous adipose tissue, show that, general, all samples highly cytocompatible.