One Step Histological Detection and Staining of the PTEN Tumor Suppressor Protein by a Single Strand DNA

• DOI: 10.3390/diagnostics11020171
• Publication Date: 2021-01-26T05:38:16Z
• Authors: Gloria Longinotti; Gabriel Ybarra; Susana Vighi; Claudia Perandones; Javier Montserrat; Juan Sebastian Ya...; Mariano Grasselli; Martin Radrizzani
• Keywords: Deoxyribozyme; Aptamer; Hemin

Antibodies are the most used technological tool in histochemistry. However, even with monoclonal antibodies, their standardization is difficult due to variation of biological systems as well variability affinity and amplification signal arising from secondary peroxidase detection systems. In this article we combined two synthetic molecules facilitate a protocol protein markers histological sections. The first molecule was an aptamer, 50-base single-stranded DNA fragment, which recognizes PTEN tumor suppressor. second also another single stranded 18-base aptamer forms quadruplex structure guanine box. This G-quadruplex attaches hemin, increasing catalytic capacity for hydrogen peroxide. Our results show how correct structural design combining together peroxidase-like DNAzyme allows detect proteins offers prognostic cancer, quality quantity, its nature 1:1 antigen:enzyme ratio. time that reproducible have been presented sections staining cancer marker using dual function.