HMGB1 Carried by Small Extracellular Vesicles Potentially Plays a Role in Promoting Acquired Middle Ear Cholesteatoma

• DOI: 10.3390/diagnostics13223469
• Publication Date: 2023-11-17T14:23:43Z
• Authors: Michał W. Łuczak; Karolina Dżaman; Łukasz Zaręba; Katarzyna Czerwaty; Jacek Siewiera; Alicja Głuszko; Ewa Olszewska; Jacek Brzost; Ireneusz Kantor; Mirosław J. Szcze...; Nils Ludwig
• Keywords: HMGB1; Pathogenesis; RAGE

Cholesteatoma is a specific medical condition involving the abnormal, non-cancerous growth of skin-like tissue in middle ear, potentially leading to collection debris and even infections. The receptor for advanced glycation (RAGE) its ligand, high-mobility box 1 (HMGB1), are both known be overexpressed cholesteatoma play potential role pathogenesis disease. In this study, we investigated small extracellular vesicles (sEVs) carrying HMGB1 inducing disease-promoting effects cholesteatoma. No significant differences concentration isolated sEVs plasma patients (n = 17) controls 22) were found (p > 0.05); however, cholesteatoma-derived carried significantly higher levels < 0.05). comparison from controls, enhanced keratinocyte proliferation IL-6 production 0.05), by engaging multiple activation pathways including MAPKp44/p42, STAT3, NF-κB pathway. Thus, HMGB1(+) emerge as novel factor promoting progression.