Method for Malaria Diagnosis Based on Extractions of Samples Using Non-Invasive Techniques: An Opportunity for the Nursing Clinical Practice

• DOI: 10.3390/ijerph17155551
• Publication Date: 2020-08-03T10:16:47Z
• Authors: Adela Gómez-Luque; Juan Carlos Parejo; Maria Zoraida Cla...; Fidel López-Espuela; Faustin Munyaruguru; Silvia Belinchón ...; Isabel Monroy; Luis Carlos Gómez...
• Keywords: Diagnosis of malaria; Plasmodium (life cycle); Dried blood

Malaria has been for millennia one of the best known and most destructive diseases affecting humans. Its high impact aroused great interest development new effective reliable diagnostic techniques. Recently it recently published that hairs from mammal hosts are able to capture, hold finally remove foreign DNA sequences Leishmania parasites. The aim this study was check if Plasmodium falciparum (P. falciparum) remains stable in blood samples deposited Whatman paper after suffering different transport storage conditions, compare sensitivity these results with those offered by thick a smear Rapid Diagnostic Test, besides examine whether P. would be detected quantified Real time quantitative PCR (qPCR) people types malaria. Histidine Repeat Protein II (pHRP-II) antigen detection were 18 19 dry adhered (94.74%), besides, also seven out hair analyzed (36.84%), remaining until analysis several months under exposure environmental conditions. Although diagnosis malaria is not as analysis, presence could constitute complementary tool numerous advantages sample collection, storage. We suggest method applied medical, forensic paleo-parasitological diagnosis, only but searching many other pathogens samples.