Biochemical Properties of a Decoy Oligodeoxynucleotide Inhibitor of STAT3 Transcription Factor

• DOI: 10.3390/ijms19061608
• Publication Date: 2018-05-30T08:03:24Z
• Authors: David S. Lee; Rachel A. O’Keefe; Patrick K. Ha; Jennifer R. Grandis; Daniel E. Johnson
• Keywords: Decoy; Transcription

Cyclic STAT3 decoy (CS3D) is a second-generation, double-stranded oligodeoxynucleotide (ODN) that mimics genomic response element for signal transducer and activator of transcription 3 (STAT3), an oncogenic factor. CS3D competitively inhibits binding to target gene promoters, resulting in decreased expression proteins promote cellular proliferation survival. Previous studies have demonstrated antitumor activity preclinical models solid tumors. However, prior entering human clinical trials, the efficiency generating molecule its stability biological fluids should be determined. synthesized as single-stranded ODN must free ends ligated generate final cyclic form. In this study, we report ligation nearly 95 percent. The half-life 7.9 h serum, indicating adequate intravenous delivery. These results provide requisite biochemical characterization will inform upcoming trials.