Proteomics-Metabolomics Combined Approach Identifies Peroxidasin as a Protector against Metabolic and Oxidative Stress in Prostate Cancer

• DOI: 10.3390/ijms20123046
• Publication Date: 2019-06-21T15:54:31Z
• Authors: Jodi Dougan; Ohuod Hawsawi; Liza J. Burton; Gabrielle Edwards; Kia Jones; Jin Zou; Peri Nagappan; Guangdi Wang; Qiang Zhang; Alira Danaher; Nathan Bowen; Cimona Hinton; Valerie A. Odero-...
• Keywords: Male; Proteomics; 0301 basic medicine; Extracellular Matrix Proteins; Peroxidasin; apoptosis; Gluconeogenesis; Prostatic Neoplasms; Apoptosis; PXDN; prostate cancer; Article; 3. Good health; Oxidative Stress; 03 medical and health sciences; Cell Line, Tumor; oxidative stress; Humans; Metabolomics; metabolome; Peroxidase

Peroxidasin (PXDN), a human homolog of Drosophila PXDN, belongs to the family heme peroxidases and has been found promote oxidative stress in cardiovascular tissue, however, its role prostate cancer not previously elucidated. We hypothesized that PXDN promotes progression via regulation metabolic pathways. analyzed expression tissue by immunohistochemistry increased with as compared normal or cells. knockdown followed proteomic analysis revealed an increase stress, mitochondrial dysfunction gluconeogenesis Additionally, Liquid Chromatography tandem mass spectrometry (LC-MS/MS)-based metabolomics confirmed induced global reprogramming associated decreased nucleotide biosynthesis. further demonstrated led reactive oxygen species (ROS) cell viability apoptosis. Finally, colony formation on soft agar. Overall, data suggest regulating metabolome, more specifically, inhibiting leading Therefore, may be biomarker potential therapeutic target.