Transcriptomic Analysis of Ficus carica Peels with a Focus on the Key Genes for Anthocyanin Biosynthesis

• DOI: 10.3390/ijms21041245
• Publication Date: 2020-02-14T15:09:57Z
• Authors: Jing Li; Yuyan An; Liangju Wang
• Keywords: Moraceae; Carica; Ficus; MYB; Structural gene

Fig (Ficus carica L.), a deciduous fruit tree of the Moraceae, provides ingredients for human health such as anthocyanins. However, little information is available on its molecular structure. In this study, fig peels in yellow (Y) and red (R) stages were used transcriptomic analyses. Comparing R with Y stage, we obtained 6224 differentially expressed genes, specifically, anthocyanin-related genes including five CHS, three CHI, DFR, ANS, two UFGT seven R2R3-MYB genes. Furthermore, anthocyanin biosynthetic i.e., FcCHS1, FcCHI1 FcDFR1, FcMYB21 FcMYB123, cloned; sequences analysis their characteristics indicated important roles biosynthesis. Heterologous expression FcMYB123 significantly promoted accumulation both apple fruits calli, further suggesting regulatory coloration. These findings provide novel insights into mechanisms behind biosynthesis coloration, facilitating genetic improvement high-anthocyanin cultivars other horticultural traits fruits.