Functional Aspects of Early Light-Induced Protein (ELIP) Genes from the Desiccation-Tolerant Moss Syntrichia caninervis

• DOI: 10.3390/ijms21041411
• Publication Date: 2020-02-20T08:20:03Z
• Authors: Xiujin Liu; Yigong Zhang; Honglan Yang; Yuqing Liang; Xiaoshuang Li; Melvin J. Oliver; Daoyuan Zhang
• Keywords: Chlorophyll; Chloroplasts; Genotype; Light; Ultraviolet Rays; high-light; Arabidopsis; Germination; ScELIP; Bryophyta; Article; 03 medical and health sciences; Gene Expression Regulation, Plant; Stress, Physiological; Desiccation; Photosynthesis; Phylogeny; Plant Proteins; 0303 health sciences; photosynthesis; Arabidopsis Proteins; 15. Life on land; Plants, Genetically Modified; photoprotection; Phenotype; Seedlings; Germ Cells, Plant

The early light-induced proteins (ELIPs) are postulated to act as transient pigment-binding proteins that protect the chloroplast from photodamage caused by excessive light energy. Desert mosses such as Syntrichia caninervis, that are desiccation-tolerant and homoiochlorophyllous, are often exposed to high-light conditions when both hydrated and dry ELIP transcripts are accumulated in response to dehydration. To gain further insights into ELIP gene function in the moss S. caninervis, two ELIP cDNAs cloned from S. caninervis, ScELIP1 and ScELIP2 and both sequences were used as the basis of a transcript abundance assessment in plants exposed to high-light, UV-A, UV-B, red-light, and blue-light. ScELIPs were expressed separately in an Arabidopsis ELIP mutant Atelip. Transcript abundance for ScELIPs in gametophytes respond to each of the light treatments, in similar but not in identical ways. Ectopic expression of either ScELIPs protected PSII against photoinhibition and stabilized leaf chlorophyll content and thus partially complementing the loss of AtELIP2. Ectopic expression of ScELIPs also complements the germination phenotype of the mutant and improves protection of the photosynthetic apparatus of transgenic Arabidopsis from high-light stress. Our study extends knowledge of bryophyte photoprotection and provides further insight into the molecular mechanisms related to the function of ELIPs.