Characterization of Naturally Occurring Bioactive Factor Mixtures for Bone Regeneration

• DOI: 10.3390/ijms21041412
• Publication Date: 2020-02-20T08:20:03Z
• Authors: Henriette Bretsch...; Mandy Quade; Anja Lode; Michael Gelinsky; Stefan Rammelt; Stefan Zwingenberger; Klaus-Dieter Schaser; Corina Vater
• Keywords: Angiogenin

In this study, the bone-regenerative potential of bioactive factors derived from adipose tissue, platelet-rich plasma (PRP) and conditioned medium hypoxia-treated human telomerase immortalized bone-marrow-derived mesenchymal stem cells (hTERT-MSC) was investigated in vitro with aim to develop cost-effective efficient bone substitutes for optimized regeneration defects. Adipose tissue harvested donors undergoing reconstructive surgery, extract (ATE) prepared. Platelet lysates (PL) were produced by repeated freeze-thaw cycles PRP, hypoxia-conditioned (HCM) obtained culturing stromal 5 days 1% O2. Besides analysis cytokine angiogenesis arrays, ELISA performed. Angiogenic cocultures (BM)-MSC umbilical vein endothelial cells. Multiple angiogenic proteins cytokines detected all growth factor mixtures. HCM ATE contained high amounts angiogenin CCL2/MCP-1, whereas PL IGFBP-1. Culturing significantly increased specific ALP activity BM-MSC as well tubule length junctions networks, indicating osteogenic stimulation. To achieve a synergism between chemoattractive differentiation capacity, combination different appears promising clinical applications.