Exposure of Triclosan in Porcine Oocyte Leads to Superoxide Production and Mitochondrial-Mediated Apoptosis during In Vitro Maturation
Dichlorofluorescein
In vitro maturation
Mitochondrial ROS
DOI:
10.3390/ijms21093050
Publication Date:
2020-04-28T09:05:32Z
AUTHORS (6)
ABSTRACT
While triclosan (TCS) exerts detrimental effects on female reproduction, the effect of TCS-derived toxins porcine oocytes during in vitro maturation (IVM) is unclear. This study investigated TCS mitochondrion-derived reactive oxygen species (ROS) production and apoptosis pathways oocyte maturation. Porcine were treated with (1, 10, 100 μM) triphenylphosphonium chloride (Mito-TEMPO; 0.1 μM), matured cumulus complexes (COCs) stained orcein, dichlorofluorescein diacetate (DCF-DA), Mito-SOX. Proteins mRNA levels factors related to expansion mitochondrion-mediated antioxidant enzymes analyzed by western blotting reverse-transcription polymerase chain reaction (RT-PCR), respectively. Meiotic cell significantly decreased for COCs after treatment along an increase mitochondrial superoxide at 44 h IVM. Further, mitochondrion-related markers elevated following TCS-mediated oxidative damage. The protective Mito-TEMPO as a specific scavenger from toxin improved capacity COCs. downregulated TCS-exposed reducing level. In conclusion, our data demonstrate that mediates toxicity through apoptosis.
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