Exposure of Triclosan in Porcine Oocyte Leads to Superoxide Production and Mitochondrial-Mediated Apoptosis during In Vitro Maturation

• DOI: 10.3390/ijms21093050
• Publication Date: 2020-04-28T09:05:32Z
• Authors: Hyo-Jin Park; Bong-Seok Song; Jin-Woo Kim; Seul-Gi Yang; Sun-Uk Kim; Deog-Bon Koo
• Keywords: 0301 basic medicine; Cumulus Cells; triclosan; Mito-TEMPO; Swine; apoptosis; Apoptosis; Article; Triclosan; In Vitro Oocyte Maturation Techniques; Mitochondria; Oxidative Stress; 03 medical and health sciences; Organophosphorus Compounds; Gene Expression Regulation; Piperidines; Superoxides; Oocytes; Animals; Female; superoxide; cumulus cell expansion; porcine oocyte maturation

While triclosan (TCS) exerts detrimental effects on female reproduction, the effect of TCS-derived toxins on porcine oocytes during in vitro maturation (IVM) is unclear. This study investigated the effects of TCS on mitochondrion-derived reactive oxygen species (ROS) production and apoptosis pathways during porcine oocyte maturation. Porcine oocytes were treated with TCS (1, 10, and 100 μM) and triphenylphosphonium chloride (Mito-TEMPO; 0.1 μM), and matured cumulus oocyte complexes (COCs) were stained with orcein, dichlorofluorescein diacetate (DCF-DA), and Mito-SOX. Proteins and mRNA levels of factors related to cumulus expansion and mitochondrion-mediated apoptosis and antioxidant enzymes were analyzed by western blotting and reverse-transcription polymerase chain reaction (RT-PCR), respectively. Meiotic maturation and cumulus cell expansion significantly decreased for COCs after TCS treatment along with an increase in mitochondrial superoxide levels at 44 h of IVM. Further, mitochondrion-related antioxidant enzymes and apoptosis markers were significantly elevated in porcine COCs following TCS-mediated oxidative damage. The protective effect of Mito-TEMPO as a specific superoxide scavenger from TCS toxin improved the maturation capacity of porcine COCs. Mito-TEMPO downregulated the mitochondrial apoptosis of TCS-exposed porcine COCs by reducing superoxide level. In conclusion, our data demonstrate that TCS mediates toxicity during porcine oocyte maturation through superoxide production and mitochondrion-mediated apoptosis.