Can miRNA Indicate Risk of Illness after Continuous Exposure to M. tuberculosis?

• DOI: 10.3390/ijms22073674
• Publication Date: 2021-04-01T11:23:17Z
• Authors: Cleonardo Augusto...; Arthur Ribeiro-do...; Wanderson Gonçalv...; Pablo Pinto; Rafael Pompeu Pan...; Tatiana Vinasco-S...; André Maurício Ri...; Mara Helena Hutz; Amanda Ferreira V...; Gilderlanio Santa...; Ândrea Ribeiro-do...; Sidney Santos
• Keywords: 0301 basic medicine; Sequence Analysis, RNA; Gene Expression Profiling; High-Throughput Nucleotide Sequencing; Article; 3. Good health; MicroRNAs; 03 medical and health sciences; tuberculosis; differential expression analysis; Case-Control Studies; biochemistry; Humans; Tuberculosis; Biomarkers; miRNA

The role of regulatory elements such as small ncRNAs and their mechanisms are poorly understood in infectious diseases. Tuberculosis is one of the oldest infectious diseases of humans and it is still a challenge to prevent and treat. Control of the infection, as well as its diagnosis, are still complex and current treatments used are linked to several side effects. This study aimed to identify possible biomarkers for tuberculosis by applying NGS techniques to obtain global miRNA expression profiles from 22 blood samples of infected patients with tuberculosis (n = 9), their respective healthy physicians (n = 6) and external healthy individuals as controls (n = 7). Samples were run through a pipeline consisting of differential expression, target genes, gene set enrichment and miRNA–gene network analyses. We observed 153 altered miRNAs, among which only three DEmiRNAs (hsa-let-7g-5p, hsa-miR-486-3p and hsa-miR-4732-5p) were found between the investigated patients and their respective physicians. These DEmiRNAs are suggested to play an important role in granuloma regulation and their immune physiopathology. Our results indicate that miRNAs may be involved in immune modulation by regulating gene expression in cells of the immune system. Our findings encourage the application of miRNAs as potential biomarkers for tuberculosis.