Identification of Candidate Genes Regulating Drought Tolerance in Pearl Millet

• DOI: 10.3390/ijms23136907
• Publication Date: 2022-06-22T01:55:19Z
• Authors: Animikha Chakraborty; Aswini Viswanath; Renuka Malipatil; Janani Semalaiyappan; Priya Shah; Swarna Ronanki; Abhishek Rathore; Sumer Pal Singh; Mahalingam Govind...; Vilas A. Tonapi; Nepolean Thirunav...
• Keywords: Drought Tolerance; MYB; Candidate gene

Pearl millet is an important crop of the arid and semi-arid ecologies to sustain food fodder production. The greater tolerance drought stress attracts us examine its cellular molecular mechanisms via functional genomics approaches augment grain yield. Here, we studied response 48 inbreds representing four different maturity groups at flowering stage. A set 74 drought-responsive genes were separated into five major phylogenic belonging eight groups, namely ABA signaling, hormone ion osmotic homeostasis, TF-mediated regulation, adaptation, signal transduction, physiological detoxification, which comprehensively studied. Among conserved motifs genes, protein kinases MYB domain proteins most ones. Comparative in-silico analysis across crops showed foxtail had orthologs with pearl millet. Of 698 haplotypes identified crops, MyC2 Myb4 maximum haplotypes. protein-protein interaction network ABI2, P5CS, CDPK, DREB, MYB, CYP707A3 as hub genes. expression assay presence common well unique groups. Drought tolerant genotypes in respective from pattern several gene families, TFs, signaling prospective contributors functionally validated could be used promising candidates backcross breeding, genomic selection, gene-editing schemes other increase yield drought-prone ecologies.