Genome-Wide Analysis of the Caffeoyl Coenzyme A-O-Methyltransferase (CCoAOMT) Gene Family in Platycodon grandiflorus (Jacq.) A. DC. and the Potential Regulatory Mechanism in Response to Copper Stress

• DOI: 10.3390/ijms26104709
• Publication Date: 2025-05-14T16:22:17Z
• Authors: Junbai Ma; Shan Jiang; Lingyang Kong; Lengleng Ma; Xinxin Wang; Meitong Pan; Chenliang Li; Shumin Huang; Xiubo Liu; Wei Ma; Weichao Ren

In recent years, copper pollution has gradually become one of the major problems soil environmental pollution. Lignin plays an important role in plant resistance to biotic and abiotic stresses. CCoAOMT is a key enzyme lignin biosynthesis process. this study, gene family members Platycodon grandiflorus were identified by bioinformatics methods, their basic characteristics potential functions analyzed. The results showed that five PgCCoAOMT P. grandiflorus, with protein lengths ranging from 246 635 amino acids, evenly distributed on four chromosomes. Phylogenetic analysis indicated was divided into two subclades, namely Clade1a, Clade1b, Clade1c, Clade1d, Clade2. cis-regulatory element promoter revealed contained large number elements responsive stress, conjecture PgCCoAOMT2, PgCCoAOMT4, PgCCoAOMT5 involved synthesis. qRT-PCR that, within 5 days stress treatment, except for PgCCoAOMT4 gene, other genes exhibited different expression levels. Furthermore, levels all increased significantly at 7 treatment. With increase content seedings trend increasing first then decreasing under stress. general, treatment 1-3 days, transcriptional inhibition PgCCoAOMT1 PgCCoAOMT3 contradicted each other, suggesting there post-translational activation or alternative metabolic pathways compensation. Meanwhile, 7-day coordinated up-regulation accompanied failure synthesis, which pointed core bottleneck precursors depletion activity inactivation caused root damage. Research objective: This study reveals level providing theoretical basis elucidating mechanism response subsequent improvement grandiflorus.