The L1014F Knockdown Resistance Mutation Is Not a Strong Correlate of Phenotypic Resistance to Pyrethroids in Florida Populations of Culex quinquefasciatus

• DOI: 10.3390/insects15030197
• Publication Date: 2024-03-15T08:47:05Z
• Authors: Alden S. Estep; Neil D. Sanscrainte; Jason Stuck; Isik Unlu; Agne Prasauskas; Stephanie J. Mundis; Nicholas Cotter; Ana L. Romero-Weaver; Troy J. Fedirko; Natalie L. Kendzi...; Kyle J. Kosinski; Daviela Ramirez; Eva A. Buckner
• Keywords: Knockdown resistance; Culex quinquefasciatus

Culex quinquefasciatus is an important target for vector control because of its ability to transmit pathogens that cause disease. Most populations are resistant pyrethroids and often organophosphates, the two most common classes active ingredients used by public health agencies. A knockdown resistance (kdr) mutation, resulting in amino acid change from a leucine phenylalanine voltage gated sodium channel, one mechanism contributing pyrethroid phenotype. Enzymatic has also been shown play very role. Recent studies have strong even when kdr relatively low, which indicates factors other than may be larger contributors resistance. In this study, we examined, on statewide scale (over 70 populations), strength correlation between CDC bottle bioassay genotypes allele frequencies. Spearman analysis showed only moderate (−0.51) or weak (−0.29) genotype permethrin deltamethrin resistance, respectively. The frequency was weaker correlate genotype. These results indicate assessing not good surrogate phenotypic testing.