Cell Instructive Behavior of Composite Scaffolds in a Co-Culture of Human Mesenchymal Stem Cells and Peripheral Blood Mononuclear Cells

• DOI: 10.3390/jfb15050116
• Publication Date: 2024-04-29T08:26:16Z
• Authors: Georgia-Ioanna Ko...; Amedeo Franco Bon...; Carmelo De Maria; Raasti Naseem; Catarina Coelho; Kalliopi Alpantaki; Aristea Batsali; Charalampos Ponti...; Paulo Quadros; Kenneth Dalgarno; Giovanni Vozzi; Chiara Vitale-Bro...; Maria Chatzinikol...
• Keywords: Medicine (General); 0303 health sciences; PHBV; PLLA; Article; 3D-printed scaffolds; osteogenesis; 03 medical and health sciences; R5-920; PLLA; PCL; PHBV; 3D-printed scaffolds; osteogenesis; osteoclastogenesis; bone tissue engineering; bioactive scaffolds; strontium; nanohydroxyapatite; PCL; osteoclastogenesis; TP248.13-248.65; Biotechnology

The in vitro evaluation of 3D scaffolds for bone tissue engineering mono-cultures is a common practice; however, it does not represent the native complex nature tissue. Co-cultures osteoblasts and osteoclasts, without addition stimulating agents monitoring cellular cross-talk, remains challenge. In this study, growth factor-free co-culture human marrow-derived mesenchymal stem cells (hBM-MSCs) peripheral blood mononuclear (hPBMCs) has been established used 3D-printed engineering. were produced from PLLA/PCL/PHBV polymeric blends, with two composite materials through 2.5% w/v nanohydroxyapatite (nHA) or strontium-substituted (Sr-nHA). Cell morphology data showed that hPBMCs remained undifferentiated co-culture, while no obvious differences observed mono- co-cultures hBM-MSCs. A significantly increased alkaline phosphatase (ALP) activity osteogenic gene expression was on Sr-nHA-containing scaffolds. Tartrate-resistant acid (TRAP) osteoclastogenic displayed suppressed levels Interestingly, indicated delay osteoclasts formation, as evidenced TRAP expression, demonstrating strontium acts an osteoclastogenesis inhibitor. This study presents effective model to evaluate regenerative capacity engineering, thus minimizing time-consuming costly vivo experiments.