Glucose Catabolite Repression Participates in the Regulation of Sialidase Biosynthesis by Antarctic Strain Penicillium griseofulvum P29

• DOI: 10.3390/jof10040241
• Publication Date: 2024-03-25T16:40:11Z
• Authors: Radoslav Abrashev; Ekaterina Krumova; Penka Petrova; Rumyana Eneva; Vladislava Dishli...; Yana Gocheva; Stefan Engibarov; Jeny Miteva-Staleva; Boryana Spasova; Vera Kolyovska; Maria Angelova
• Keywords: Sialidase

Sialidases (neuraminidases) catalyze the removal of terminal sialic acid residues from glycoproteins. Novel enzymes non-clinical isolates are increasing interest regarding their application in food and pharmaceutical industry. The present study aimed to evaluate participation carbon catabolite repression (CCR) regulation cold-active sialidase biosynthesis by psychrotolerant fungal strain Penicillium griseofulvum P29, isolated Antarctica. presence glucose inhibited activity growing non-growing mycelia a dose- time-dependent manner. same response was demonstrated with maltose sucrose. replacement glucose-6-phosphate also exerted CCR. addition cAMP resulted partial de-repression synthesis. CCR P. P29 did not depend on temperature. Sialidase might be subject both at 10 25 °C. fluorescent assay using 4MU-Neu5Ac for enzyme determination under concentrations evidenced that may have regulatory role production. real-time RT-PCR experiments revealed gene repression. To our knowledge, this is first report has studied effect sialidase, produced an Antarctic strain.