The Nearly Complete Genome of Grifola frondosa and Light-Induced Genes Screened Based on Transcriptomics Promote the Production of Triterpenoid Compounds

• DOI: 10.3390/jof11040322
• Publication Date: 2025-04-18T12:24:07Z
• Authors: Yang Yang; Xuebo Hu
• Keywords: Grifola frondosa; Secondary metabolite; Secondary metabolism; Sequence assembly

Grifola frondosa, commonly known as turkey tail, is a valuable fungus with medicinal and culinary uses, rich in bioactive compounds like triterpenoid polysaccharides that contribute to health benefits. Here, we constructed nearly complete genome of G. frondosa CH1 using Illumina, PacBio HiFi, Hi-C sequencing technologies, resulting 35.74 Mb 12,526 protein-coding genes. The spans 12 chromosomes, all intact telomeric structures no gaps. BUSCO completeness scores 95.1% 99.1% for the genes, respectively, indicate high assembly quality gene prediction. Phylogenetic analysis showed close relationship between Trametes cinnabarina. Transcriptomic under varying light conditions changes expression especially those related terpenoid synthesis, several CAZymes CYP450 genes also exhibiting light-induced variations. Ten secondary metabolite clusters were identified, three which light-sensitive, indicating exposure regulates metabolism. This study provides data supporting high-quality offers new insights into regulation its