Isolation and Characterization of Nano-Hydroxyapatite from Salmon Fish Bone

• DOI: 10.3390/ma8085253
• Publication Date: 2015-08-21T14:38:09Z
• Authors: Jayachandran Venk...; Baboucarr Lowe; Panchanathan Mani...; Kyong-Hwa Kang; Elna Chalisserry; Sukumaran Anil; Dong Kim; Se-Kwon Kim
• Keywords: fish; 2500 Materials Science; hydroxyapatite; salmon; hydroxyapatite; salmon; fish; alkaline hydrolysis; 540; 01 natural sciences; Article; Hydroxyapatite; 0104 chemical sciences; Alkaline hydrolysis; Fish; Salmon; 669; alkaline hydrolysis

Nano-Hydroxyapatite (nHA) was isolated from salmon bone by alkaline hydrolysis. The resulting nHA was characterized using several analytical tools, including thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), to determine the purity of the nHA sample. The removal of organic matter from the raw fish was confirmed by TGA. FT-IR confirmed the presence of a carbonated group and the similarities to synthetic Sigma HA. XRD revealed that the isolated nHA was amorphous. Microscopy demonstrated that the isolated nHA possessed a nanostructure with a size range of 6–37 nm. The obtained nHA interacted with mesenchymal stem cells (MSCs) and was non-toxic. Increased mineralization was observed for nHA treated MSCs compared to the control group. These results suggest that nHA derived from salmon is a promising biomaterial in the field of bone tissue engineering.