Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.

• DOI: 10.3390/md16010014
• Publication Date: 2018-01-08T09:21:21Z
• Authors: Hee Shin; Byeoung-Kyu Choi; Phan Trinh; Hwa-Sun Lee; Jong Kang; Tran Van; Hyi-Seung Lee; Jong Lee; Yeon-Ju Lee; Jihoon Lee
• Keywords: Sponge

A new α-pyrone merosesquiterpenoid possessing an angular tetracyclic carbon skeleton, ochraceopone F (1), and four known secondary metabolites, aspertetranone D (2), cycloechinulin (3), wasabidienone E (4), mactanamide (5), were isolated from the marine fungus Aspergillus flocculosus derived a sponge Stylissa sp. collected in Vietnam. The structures of Compounds 1–5 elucidated by analysis 1D 2D NMR spectra MS data. All compounds evaluated for anti-proliferation activity their suppression effects on receptor activator nuclear factor κB ligand (RANKL)-induced osteoclast differentiation using tartate-resisant acid phosphatase (TRAP). had no anti-proliferative effect human cancer cell lines up to 30 μg/mL. Among these compounds, (2) (4) exhibited weak inhibitory at 10 However, (5) showed potent without any evidence cytotoxicity.