In Silico Genomic Fingerprints of the Bacillus anthracis Group Obtained by Virtual Hybridization

• DOI: 10.3390/microarrays4010084
• Publication Date: 2015-02-17T15:11:14Z
• Authors: Hueman Jaimes-Díaz; Violeta Larios-Se...; Teresa Lloret-Sán...; Gabriela Olguín-Ruiz; Carlos Sánchez-Va...; Luis Carreño-Durán; Rogelio Maldonado...; Alfonso Méndez-Te...
• Keywords: Comparative genomic hybridization; genomic DNA; Bacillus anthracis

In this study we evaluate the capacity of Virtual Hybridization to identify between highly related bacterial strains. Eight genomic fingerprints were obtained by virtual hybridization for Bacillus anthracis genome set, and a set 15,264 13-nucleotide short probes designed produce unique each organism. The data from fingerprint used obtain patterns simulating DNA microarray. Two methods used: Direct Extended method number potential sites thus determine minimum sensitivity value discriminate genomes with 99.9% similarity. Genomic compared using both phylogenomic trees constructed verify that detection is 0.000017. Results suggest distribution in correct, as other taxonomic methods. Specific studied also identified.