Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.

• DOI: 10.3390/microorganisms10040704
• Publication Date: 2022-03-25T03:31:43Z
• Authors: Ivo Sedláček; Pavla Holochová; Hans-Jürgen Busse; Vendula Koublová; Stanislava Králová; Pavel Švec; Roman Sobotka; Eva Staňková; Jan Pilný; Ondrej Šedo; Jana Smolíková; Karel Sedlář
• Keywords: Psychrophile

A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part James Ross Island and Eagle Island, Antarctica, 2017–2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, exhibited strong hydrolytic activities. To clarify the taxonomic position these isolates, polyphasic approach applied based sequencing 16S rRNA, gyrB lepA genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses biotyping. Phylogenetic analysis rRNA gene sequences revealed that entire are representatives genus Massilia. closest relatives reference strain P8398T Massilia atriviolacea, violaceinigra, rubra, mucilaginosa, aquatica, frigida, glaciei eurypsychrophila with pairwise similarity 98.6–100% rRNA. subsequent results showed novelty analysed group, average nucleotide identity digital DNA–DNA hybridisation values clearly proved represents distinct species. After all results, we nominate new species proposed name antarctica sp. nov. type is (= CCM 8941T = LMG 32108T).