Metagenomic Analysis of Bacterial, Archaeal and Fungal Diversity in Two-Stage Anaerobic Biodegradation for Production of Hydrogen and Methane from Corn Steep Liquor

• DOI: 10.3390/microorganisms11051263
• Publication Date: 2023-05-11T07:22:19Z
• Authors: Galina Stoyancheva; Lyudmila Kabaivanova; Venelin Hubenov; Elena Chorukova
• Keywords: Acetogenesis; Acidogenesis; Microbial consortium

The main purpose of this study was to identify the microbial communities (bacterial, archaeal and fungal) in a two-stage system anaerobic bioreactors for production hydrogen methane from waste substrate-corn steep liquor. Wastes food industry are valuable resources with potential biotechnological because their high organic matter contents. In addition, methane, volatile fatty acids, reducing sugars cellulose content monitored. Two-stage biodegradation processes were performed by populations first generating bioreactor (working volume 3 dm3) second methane-generating reactor 15 dm3). Cumulative yield reached 2000 cm3 or 670 cm3/L day, while maximum quantity 3300 220 day. Microbial consortia digestion systems play an essential role process optimization biofuel enhancement. obtained results showed possibility conducting two separate processes-the hydrogenic (hydrolysis acidogenesis) methanogenic (acetogenesis methanogenesis)-as stages favor energy under controlled conditions corn diversity microorganisms as participants followed using metagenome sequencing bioinformatics analysis. metagenomic data that most abundant phylum both bacterial Firmicutes-58.61% 36.49% 1 2, respectively. Phylum Actinobacteria found significant quantities (22.91%) community Bioreactor 1, whereas they 2.1%. Bacteroidetes present bioreactors. Euryarchaeota made up 0.4% contents 11.4% second. As dominant genera among archaea Methanothrix (8.03%) Methanosarcina (3.39%), fungal representatives Saccharomyces cerevisiae. New knowledge mediated novel could be widely used convert different wastes green energy.